Seeking Volunteers for Seedling Inoculations @ PSU Greenhouses

PSU Greenhouse April 16 (3)Get involved with the science!

Seedling Inoculations — Greenhouses at Penn State

Thursday, June 8th  -Saturday, June 10th

Time: 9:00 am to 4:00 pm
Location: 206 Forest Resource Lab, University Park, PA 16802

Description:Across three days, Thursday, June 8 – Saturday, June 10, we will be inoculating seedling chestnuts in the greenhouses at Penn State.

We will be following the “small stem assay” (SSA) protocol developed by researchers at SUNY-ESF. This process is very similar to inoculating trees in the field, but works on much smaller trees and with much smaller wounds on the trees. Here’s the general process:

– Wound the tree with ~1mm long slash using a knit picker (
– Place a small amount of chestnut blight, growing on PDA, onto a piece of parafilm
– Wrap the parafilm around the stem, ensuring the fungus makes and keeps contact with the wound.
– We will do this two times for each tree – once for a weak strain of the fungus and once for a strong strain.

We have about 2500 trees to do. At 2 wounds each, we’ll be doing this about 5000 times.

We’d like to get about 8-10 people each day, if possible. That way we can split up into two teams and each person has a specific task the whole time.

If you can join us, please let me know which day and for what length of time.

If you have any questions or need any further information, please don’t hesitate to get in touch.

Sara Fern Fitzsimmons
Director of Restoration – TACF®
The Pennsylvania State University
206 Forest Resources Lab
University Park, PA 16802

Office: 814-863-7192


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